Ischemia-reperfusion injury (I/R) is a phenomenon whereby cellular damage in a hypoxic organ is accentuated following the restoration of oxygen delivery. Wistar rats of either sex (250-300 gm) were treated for 15 days with alcoholic extract of Berberis aristata (250mg/kg-500mg/kg) prior to ischemic reperfusion. After 15 days ischemia was produced by clamping hepatic artery and hepatic portal vein for 25 minutes and reperfusion for 40 minutes by unclamping. Liver was dissected out to prepare homogenate to measure level of Malondialdehyde, Superoxide dismutase, Catalase, Vitamin C and Protein estimation, Serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP). Animals subjected to ischemic reperfusion without any treatment were included as model control and animals treated with Silymarin were included as a standard. Animals subjected to ischemic reperfusion without any treatment showed higher level of oxidative enzymes and lower level of protective enzymes as compare to the animals treated with alcoholic extract of Berberis aristata roots. In the histopathology there was a protection against the injury produced by ischemic reperfusion in the animals treated with alcoholic extract of Berberis aristata.
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